Gel filtration chromatography is also known as molecular sieve chromatography or size exclusion chromatography. When the liquid flows through the porous filler, it is separated and purified according to the difference in physical size of each component in the separated sample. It is mainly separated according to the size and shape of the sample. Generally, large molecules flow out first and small molecules flow out later. Product features: uniform particle size, high flow rate, high resolution, good stability, and less non-specific adsorption.

Affinity chromatography is a chromatography method that fixes specific ligands on solid phase carriers and uses the specific binding between biological molecules and ligands to separate components that specifically bind to ligands from complex solution systems. For example, chromatographic media such as Ni NTA Beads 6FF, Glutathione Beads 6FF, and rProtein A Beads 4FF, respectively, fix Ni2+, GSH, and rProtein A on agarose microspheres, which can purify proteins that specifically bind to ligands.

Ion exchange chromatography is the most commonly used method for separation and purification of biomacromolecules. Its principle is to separate proteins based on their different charges under certain pH conditions. Ion exchange chromatography media based on agarose gel retain the excellent hydrophilicity and pore structure of natural polysaccharide compounds, and have good compatibility with bioactive macromolecules. They are particularly suitable for the separation and purification of proteins, enzymes, polysaccharides, nucleic acids, plasmids, etc.

Hydrophobic interaction chromatography (HIC) uses the reversible interaction between proteins and the hydrophobic surface of hydrophobic chromatography media to separate proteins according to the different hydrophobicity of the protein surface. Hydrophobic chromatography media use highly cross-linked agarose gel as the matrix and pyrenyl, butyl, etc. as ligands. They have strong hydrophobicity and high loading capacity, and are particularly suitable for the separation and purification of proteins, enzymes, polysaccharides, nucleic acids, plasmids, etc.

Multimodal chromatography is a chromatographic separation method in which more than one interaction exists between the stationary phase and the analyte. These techniques utilize a media support with functionalized ligands that simultaneously exhibit different modes of interaction, such as size exclusion, affinity, ion exchange, or hydrophobic interaction. Combining these interactions can improve selectivity during protein purification processes.

Agarose matrix magnetic microspheres (Magarose Beads) series products have the characteristics of superparamagnetism, rapid magnetic responsiveness, rich hydroxyl functional groups and relatively concentrated particle size, and are important carrier tools in medical and molecular biology research. Changzhou Tiandirenhe Biological uses a unique production process technology to prepare magnetic agarose microspheres with a particle size distribution of 30-100μm. The particle size is moderate and more suitable for the needs of biological inspection and purification experiments.

Tiandirenhe Biosciences provides a variety of supporting protein purification and related reagents for subsequent applications.

There are many different scales of high-throughput purification equipment with 10 channels, 24 channels, 32 channels and 96 channels. You can choose the appropriate equipment for purification according to different purification media, sample quantity and other requirements. The high-throughput equipment has high uniformity in each channel, which reduces manual operation time and operation errors.

Smart-lifesciences provides a variety of consumables and accessories for protein purification and subsequent applications.